The Guinness World Record for the largest litter is 24 puppies. They were born in Cambridgeshire in 2004.A guide dog has given birth to a record litter of 16 puppies. In November 2004, a Neapolitan mastiff named Tia broke the world record for the largest litter of dogs when she gave birth to 24 puppies. Despite being Unity's first pregnancy, her litter is more than twice the average size of German Shepherds and more than three times the average litter size across all breeds.
The previous title of Australia's largest litter of puppies was shared by Melody the Dalmatian in Albury and Minnie the American bulldog, says the blog post, both with litters of 19 puppies. The delivery led to a team of 10 veterinarians and nurses who removed an additional 18 puppies from the Neapolitan Mastiff, bringing their total litter to 21 puppies. The title was previously awarded jointly to Melody the Dalmatian in Albury and Minnie the American bulldog, both with litters of 19 puppies. She was raised with Trigger, a six-year-old Golden Retriever who is the most prolific stallion of Guide Dogs, having had 27 previous litters and fathered an astonishing 239 puppies.
The Unity litter is twice the size of an average German Shepherd litter and three times the size of an overall dog litter. Although the new mother Shadow has missed the title of the Guinness Book of World Records (24 born to a Neapolitan mastiff in 200), she has broken the title of Australia's largest litter of puppies.